Immortalized human podocyte cell line
WitrynaOur normal human primary cells retain in vivo physiologies and custom lots are available from a diverse donor pool. ATCC hTERT-immortalized primary cells also display … WitrynaThe conditionally immortalized human podocyte cell line AB8/13 was kindly provided by M. Saleem and has been described previously [9,74]. Podocytes were cultured in the medium which contained RPMI 1640 medium with 10% fetal bovine serum, and insulin–transferrin–selenium at 33 °C. For the differentiation, podocytes were …
Immortalized human podocyte cell line
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Witryna14 lut 2015 · Mouse podocytes immortalised by transduction with a temperature sensitive large T antigen SV40 construct as previously described 45, 46, were used … WitrynaThe podocyte is a key cell in the glomerulus in health and disease: until recently, human podocytes have been difficult to study in vitro. We have developed a conditionally immortalized human podocyte cell line transfected with a temperature-sensitive simian virus 40 transgene: when the transgene is inactivated in vitro, these …
WitrynaImmortalized Primary Cells. Innoprot offer Human and animal primary cells immortalized with SV40 antigen T and other immortalization techniques. All immortalized cell lines has been characterized for specific markers and other for functional assays. Showing 1–16 of 23 results. 1 2. WitrynaIn contrast, no significant change was observed in mesangial cells after 12 h, and even after 48 h the wounds were not completely closed. Until now, conditionally …
WitrynaSteroid-resistant nephrotic syndrome (SRNS) is a frequent cause of CKD. The discovery of monogenic causes of SRNS has revealed specific pathogenetic pathways, but these monogenic causes do not explain all cases of SRNS. To identify novel monogenic WitrynaCell line name: CIHP-1: Synonyms: Conditionally Immortalized Human Podocytes-1; AB8/13; AB 8/13; hiPOD; ciPOD: Accession: CVCL_W186: Resource Identification …
WitrynaImmortalized Human Kidney Podocytes (IM-HKP) provided by Innoprot have been developed by immortalizing primary human healthy kidney podocytes with SV40 …
WitrynaATCC cell lines immortalized human podocytes ab8 13 m saleem Cell Lines Immortalized Human Podocytes Ab8 13 M Saleem, supplied by ATCC, used in … litchfields san rafaelWitrynaA breakthrough in cell biology research. Human telomerase reverse transcriptase (hTERT)–immortalized primary cells represent a breakthrough in cell biology … imperial leather advert 70sWitrynaeases. A conditionally immortalized human podocyte cell line has been developed by transfection with the temperature-sen-sitive SV40-Tgene. These cells … imperial leather fijian waterfallWitrynaA stable SPL-kd cell line of human podocytes was generated by the lentiviral shRNA transduction method and was characterized for reduced SPL mRNA and protein levels and increased S1P levels. imperial leather bubbleworksWitryna1 mar 2010 · The generation of podocytes cell lines from urine of patients and healthy volunteers is novel and will help to advance studies of podocyte cell biology, and further improvements in the approaches to cell transformation and/or cell culture techniques are needed to allow cultured podocytes to fully reproduce in vivo characteristics. … imperial leather cotton clouds shower gelWitrynaConditionally immortalized human podocyte WT and CRISPR/CAS9 GLA KO cell lines were differentiated at 37 °C at 70% confluency for 10 to 14 days. Growth protocol Conditionally immortalized human podocytes were let to proliferate at 33°C in RPMI-1640 medium supplemented with 10% fetal calf serum (FCS), Penicillin/Streptomycin, … litchfields phoenixWitrynaATCC cell lines immortalized human podocytes ab8 13 m saleem Cell Lines Immortalized Human Podocytes Ab8 13 M Saleem, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more litchfield station hobby