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WebJun 5, 2024 · Microbes with short generation times (i.e. 10-15 minutes) may be incubated for 12-14 hours, while microbes with long generation times (i.e., several hours) may be incubated for 72 hours or longer. Incubation: Oxygen. microbes must also be incubated with appropriate oxygen requirements. Asepsis. WebFeb 14, 2024 · Flaming the neck of bottles and test tubes This ensures that no microorganisms enter the mouth of the vessel to contaminate the culture or the medium. Passing the mouth of the bottle through a flame produces a convection current away from the opening, and helps to prevent contamination. on the border las vegas nv
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