Ceramide assay kit
WebMay 17, 2013 · Ultraviolet (UV) radiation and reactive oxygen species (ROS) impair the physiological functions of retinal pigment epithelium (RPE) cells by inducing cell apoptosis, which is the main cause of age-related macular degeneration (AMD). The mechanism by which UV/ROS induces RPE cell death is not fully addressed. Here, we observed the … WebThis study investigated the protective effects of glucocerebroside-containing buttermilk concentrated powder (GCBM) on oxidative stress and photoaging in ultraviolet B (UVB)-irradiated hairless mice. We measured antioxidant enzyme activities, collagen synthesis-related pathways, and moisturizing-related factors in the dorsal skin of mice. We …
Ceramide assay kit
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WebCell Navigator® TMR Ceramide Golgi Staining Kit provides an optimized assay method for examining the morphology of the Golgi apparatus with a fluorescence microscope. In … WebCytotoxicity Assay. The cytotoxicity of PEG-ceramide nanomicelles against N2a cells was measured using a CCK-8 kit. Briefly, N2a cells (dissociated into single cells) were seeded in 96-well plates at a density of 3×10 3 cells per well and incubated overnight. The cells were then incubated for 24 h with varying concentrations of PEG-ceramide ...
WebCell Biolabs’ Sphingomyelin Assay Kit is a simple fluorometric assay that measures the amount of sphingomyelin present in plasma or serum, tissue homogenates, or cell … WebMar 30, 2024 · Ceramides involve a family of lipids consisting of sphingosine covalently linked to a fatty acid that regulates pleiotropic cellular events, including cell differentiation, proliferation, and...
WebOct 15, 1999 · A fluorescence-based high-performance liquid chromatographic assay to determine acid ceramidase activity Acid ceramidase (N-acylsphingosine amidohydrolase) is the lysosomal enzyme required to hydrolyze the N-acyl linkage between the fatty acid and sphingosine moieties in ceramide. WebThis ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the targets. This ELISA kit for research use only, not for therapeutic or diagnostic …
WebCeramide is generated by hydrolysis of sphingomyelin by sphingomyelinase. [ 1] Specificity Mouse monoclonal clone MID 15B4 anti-Ceramide antibody recognizes free and bound ceramides. Immunogen ceramide conjugated to bovine serum albumin. Application Monoclonal Anti-Ceramide antibody produced in mouse has been used in: …
WebMar 2, 2024 · Ceramides (Cer) belong to the group of ceramide lipids within the sphingolipids. Their structure consists of a long-chain base which is linked to a fatty acid … mary ann vecchio obituaryWebFeb 21, 2024 · The CerS Assay Kit offers a number of advantages including: a short assay time, a requirement of only small amounts of biological material, and elimination of the … mary ann vecchio imageWebThe Acid Sphingomyelinase Assay Kit is an enzyme assay that measures acid sphingomyelinase activity in biological samples through the direct hydrolysis of a fluorogenic substrate. Assay sensitivity: 25 pmol Sample … data stormsWebThe Qubit Protein Assay Kits are designed to make protein quantification easy and accurate. Addressing overlapping concentration ranges, the two kits together are accurate for initial sample concentrations from 12.5 μg/mL to 20 mg/mL and exhibit low protein-to-protein variation. mary ann\u0027s deli \u0026 cateringWebTo achieve ER labeling, this kit employs a primary antibody directed against an ER-associated protein, protein disulfide isomerase (PDI), and an Alexa Fluor 488 dye–labeled secondary antibody. The Alexa Fluor 488 dye exhibits bright green fluorescence that is compatible with filters and instrument settings appropriate for fluorescein. data storm xf3 1.2 automated satellite dishWebHuman Ceramide kinase / CERK ELISA Kit. Ceramide is activated to ceramide 1-phosphate (C1P), a sphingolipid metabolite, by Ceramide kinase/CERK. Both Ceramide … mary a pulsinelli gregg ohioWebThe assay will exclusively recognize both natural and recombinant human Alpha-Galactosidase A/GLA. Target information This gene encodes a homodimeric … datastor newcastle